Upstream start codon in segment 4 of North American H2 avian influenza A viruses
Identifieur interne : 000070 ( 1957/Analysis ); précédent : 000069; suivant : 000071Upstream start codon in segment 4 of North American H2 avian influenza A viruses
Auteurs : Geneviève Girard [Pays-Bas] ; Alexander P. Gultyaev [Pays-Bas] ; René C. L. Olsthoorn [Pays-Bas]Source :
- Infection, genetics and evolution : (Print) [ 1567-1348 ] ; 2011.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
H2N2 influenza A virus was the cause of the 1957 pandemic. Due to its constant presence in birds, the H2 subtype remains a topic of interest. In this work, comparison of H2 leader sequences of influenza A segment 4 revealed the presence of an upstream in-frame start codon in a majority of North American avian strains. This AUG is located seven codons upstream of the conventional start codon and is in a good Kozak context. In vivo experiments, using a luciferase reporter gene fused to leader sequences derived from North American avian H2 strains, support the efficient use of the upstream start codon. These results were corroborated by in vitro translation data using full-length segment 4 mRNA. Phylogenic analyses indicate that the upstream AUG, first detected in 1976, is stably nested in the North American avian lineage of H2 strains nowadays. The possible consequences of the upstream AUG are discussed.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000014
- to stream PascalFrancis, to step Curation: 000060
- to stream PascalFrancis, to step Checkpoint: 000008
- to stream Main, to step Merge: 000D63
- to stream Main, to step Curation: 000D58
- to stream Main, to step Exploration: 000D58
- to stream 1957, to step Extraction: 000070
Links to Exploration step
Pascal:11-0148190Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Upstream start codon in segment 4 of North American H2 avian influenza A viruses</title><author><name sortKey="Girard, Genevieve" sort="Girard, Genevieve" uniqKey="Girard G" first="Geneviève" last="Girard">Geneviève Girard</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502</s1><s2>2300 RA Leiden</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Gultyaev, Alexander P" sort="Gultyaev, Alexander P" uniqKey="Gultyaev A" first="Alexander P." last="Gultyaev">Alexander P. Gultyaev</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502</s1><s2>2300 RA Leiden</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Virology, Erasmus Medical Center, P.O. Box 2040</s1><s2>3000 CA Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>3000 CA Rotterdam</wicri:noRegion></affiliation></author><author><name sortKey="Olsthoorn, Rene C L" sort="Olsthoorn, Rene C L" uniqKey="Olsthoorn R" first="René C. L" last="Olsthoorn">René C. L. Olsthoorn</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502</s1><s2>2300 RA Leiden</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">11-0148190</idno><date when="2011">2011</date><idno type="stanalyst">PASCAL 11-0148190 INIST</idno><idno type="RBID">Pascal:11-0148190</idno><idno type="wicri:Area/PascalFrancis/Corpus">000014</idno><idno type="wicri:Area/PascalFrancis/Curation">000060</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000008</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000008</idno><idno type="wicri:doubleKey">1567-1348:2011:Girard G:upstream:start:codon</idno><idno type="wicri:Area/Main/Merge">000D63</idno><idno type="wicri:Area/Main/Curation">000D58</idno><idno type="wicri:Area/Main/Exploration">000D58</idno><idno type="wicri:Area/1957/Extraction">000070</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Upstream start codon in segment 4 of North American H2 avian influenza A viruses</title><author><name sortKey="Girard, Genevieve" sort="Girard, Genevieve" uniqKey="Girard G" first="Geneviève" last="Girard">Geneviève Girard</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502</s1><s2>2300 RA Leiden</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Gultyaev, Alexander P" sort="Gultyaev, Alexander P" uniqKey="Gultyaev A" first="Alexander P." last="Gultyaev">Alexander P. Gultyaev</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502</s1><s2>2300 RA Leiden</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Virology, Erasmus Medical Center, P.O. Box 2040</s1><s2>3000 CA Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>3000 CA Rotterdam</wicri:noRegion></affiliation></author><author><name sortKey="Olsthoorn, Rene C L" sort="Olsthoorn, Rene C L" uniqKey="Olsthoorn R" first="René C. L" last="Olsthoorn">René C. L. Olsthoorn</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502</s1><s2>2300 RA Leiden</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Infection, genetics and evolution : (Print)</title><idno type="ISSN">1567-1348</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Infection, genetics and evolution : (Print)</title><idno type="ISSN">1567-1348</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Avian influenza</term><term>Codon</term><term>Hemagglutinin</term><term>Influenza A</term><term>Influenza A virus</term><term>Molecular epidemiology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Grippe aviaire</term><term>Grippe A</term><term>Codon</term><term>Hémagglutinine</term><term>Virus grippal A</term><term>Epidémiologie moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">H2N2 influenza A virus was the cause of the 1957 pandemic. Due to its constant presence in birds, the H2 subtype remains a topic of interest. In this work, comparison of H2 leader sequences of influenza A segment 4 revealed the presence of an upstream in-frame start codon in a majority of North American avian strains. This AUG is located seven codons upstream of the conventional start codon and is in a good Kozak context. In vivo experiments, using a luciferase reporter gene fused to leader sequences derived from North American avian H2 strains, support the efficient use of the upstream start codon. These results were corroborated by in vitro translation data using full-length segment 4 mRNA. Phylogenic analyses indicate that the upstream AUG, first detected in 1976, is stably nested in the North American avian lineage of H2 strains nowadays. The possible consequences of the upstream AUG are discussed.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Girard, Genevieve" sort="Girard, Genevieve" uniqKey="Girard G" first="Geneviève" last="Girard">Geneviève Girard</name></noRegion><name sortKey="Gultyaev, Alexander P" sort="Gultyaev, Alexander P" uniqKey="Gultyaev A" first="Alexander P." last="Gultyaev">Alexander P. Gultyaev</name><name sortKey="Gultyaev, Alexander P" sort="Gultyaev, Alexander P" uniqKey="Gultyaev A" first="Alexander P." last="Gultyaev">Alexander P. Gultyaev</name><name sortKey="Olsthoorn, Rene C L" sort="Olsthoorn, Rene C L" uniqKey="Olsthoorn R" first="René C. L" last="Olsthoorn">René C. L. Olsthoorn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/1957/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000070 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/1957/Analysis/biblio.hfd -nk 000070 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= 1957
|étape= Analysis
|type= RBID
|clé= Pascal:11-0148190
|texte= Upstream start codon in segment 4 of North American H2 avian influenza A viruses
}}
| This area was generated with Dilib version V0.6.33. |  |